3-D Imaging System

ApoTome is a 3-D imaging system for contrast enhancement in fluorescence microscopy based on innovative grid projection techniques using HBO light source. This new technique permits optical sections to be produced quickly and with very good quality. It dramatically improves clarity and performance level of digital imaging workstations in neuroscience, cell and developmental biology and can be used with a variety of fluorochromes such as DAPI, FITC, Rhodamine, GFP or YFP. To address the challenges in capturing optical sections in 2D and 3D fluorescence imaging, particularly of thick specimens, the slider is inserted into the plane of the field diaphragm of the fluorescence beam path of the Zeiss Axioplan 2 IE or Axiovert 200 microscopes. No stray light from other focal planes, time-consuming reconstructions or long calculations and post-processing. Furthermore, optical sections are a major requirement for 3D reconstruction.