Research & Development World

  • R&D World Home
  • Topics
    • Aerospace
    • Automotive
    • Biotech
    • Careers
    • Chemistry
    • Environment
    • Energy
    • Life Science
    • Material Science
    • R&D Management
    • Physics
  • Technology
    • 3D Printing
    • A.I./Robotics
    • Software
    • Battery Technology
    • Controlled Environments
      • Cleanrooms
      • Graphene
      • Lasers
      • Regulations/Standards
      • Sensors
    • Imaging
    • Nanotechnology
    • Scientific Computing
      • Big Data
      • HPC/Supercomputing
      • Informatics
      • Security
    • Semiconductors
  • R&D Market Pulse
  • R&D 100
    • Call for Nominations: The 2025 R&D 100 Awards
    • R&D 100 Awards Event
    • R&D 100 Submissions
    • Winner Archive
    • Explore the 2024 R&D 100 award winners and finalists
  • Resources
    • Research Reports
    • Digital Issues
    • R&D Index
    • Subscribe
    • Video
    • Webinars
  • Global Funding Forecast
  • Top Labs
  • Advertise
  • SUBSCRIBE

A new way to profile immune cells in blood

By R&D Editors | March 7, 2014

A new technique distinguishes cell types in blood by looking at signature degrees of methylation in DNA. Yellow represents no methylation, blue full methylation. Image: Kelsey laboratory/Brown Univ.When a person becomes sick or is exposed to an unwelcome substance, the body mobilizes specific proportions of different immune cells in the blood. Methods of discovering and detecting those profiles are therefore useful both clinically and in research. In a new Genome Biology paper, a team of scientists describes a new and uniquely advantageous way to detect them.

All the current means of counting immune cells in a blood sample require whole cells, said Karl Kelsey, prof. of epidemiology at Brown and corresponding author, but the new system relies on something far less ephemeral: DNA. Its use of hardy strands of genetic material allows it to handle even archived samples where cells have lost their physical integrity.

All of a person’s immune cells—in fact, nearly all of their cells—have exactly the same DNA, but what makes a kidney cell different from a brain cell or a T-cell distinct from a B-cell are chemical alterations known as epigenetic marks. Those cause a cell’s genes to be expressed in the particular way that makes them different. One type of those alterations is methylation, and every kind of cell has its own methylation signature.

“Once you understand the unique and really immutable signature that directs the differentiation of the cell, then you can use that and you don’t need the cell anymore,” Kelsey said.

So the new test detects those methylation signatures in a blood sample and, with the help of sophisticated algorithms, counts up how many cells of each type are in the sample. In the experiments reported in the paper, Kelsey, lead author William Accomando, a postdoctoral research fellow at the Harvard School of Public Health, and colleagues counted up the following major immune cell types, or leukocytes: T-cells, B-cells, NK cells, monocytes, eosinophils, basophils and neutrophils.

Based on their tests using fresh human blood samples from more than 80 donors, they report that their technique’s accuracy performed at par in a direct comparison with three “gold standard” methods: “manual five-part differential,” “CBC with automated five-part differential” and “fluorescence activated cell sorting.”

In further experiments they showed that their technique works to detect the mixtures of immune cells associated with known diseases and that the technique works with blood exposed to storage conditions such as freezing and the addition of anticoagulants.

Moreover, in their experiments the team showed that to distinguish among and count those various immune cell types, they only needed to measure a few dozen methylation marks in the DNA. What’s sufficient to constitute a signature, in other words, can be quite short.

The main ingredients of the method, Kelsey said, are libraries of methylation signatures of cells. Kelsey’s laboratory determined the ones needed for this study, but big new epigenetics research consortia in Europe and the U.S. are poised to produce many more, greatly expanding the versatility of the proprietary method to cover more immune cells and other cell types as well.

In addition to the algorithms and the libraries, the test also requires some hardware, such as commercially available methylation microarrays.

The method has proven feasible enough that many other epidemiology research labs are already using it, Kelsey said. Brown Univ. has also applied for a patent on the technique. He said it has the potential to be cheaper and faster than current techniques, although he didn’t measure that in the paper.

Source: Brown Univ.

Related Articles Read More >

Open-source Boltz-2 can speed binding-affinity predictions 1,000-fold
Thermo Fisher’s new Orbitrap Excedion Pro targets complex biotherapeutics for drug development
FDA’s new ‘Elsa’ AI set to expedite clinical protocol reviews
Waters touts six-fold robustness with new Xevo TQ Absolute XR
rd newsletter
EXPAND YOUR KNOWLEDGE AND STAY CONNECTED
Get the latest info on technologies, trends, and strategies in Research & Development.
RD 25 Power Index

R&D World Digital Issues

Fall 2024 issue

Browse the most current issue of R&D World and back issues in an easy to use high quality format. Clip, share and download with the leading R&D magazine today.

Research & Development World
  • Subscribe to R&D World Magazine
  • Enews Sign Up
  • Contact Us
  • About Us
  • Drug Discovery & Development
  • Pharmaceutical Processing
  • Global Funding Forecast

Copyright © 2025 WTWH Media LLC. All Rights Reserved. The material on this site may not be reproduced, distributed, transmitted, cached or otherwise used, except with the prior written permission of WTWH Media
Privacy Policy | Advertising | About Us

Search R&D World

  • R&D World Home
  • Topics
    • Aerospace
    • Automotive
    • Biotech
    • Careers
    • Chemistry
    • Environment
    • Energy
    • Life Science
    • Material Science
    • R&D Management
    • Physics
  • Technology
    • 3D Printing
    • A.I./Robotics
    • Software
    • Battery Technology
    • Controlled Environments
      • Cleanrooms
      • Graphene
      • Lasers
      • Regulations/Standards
      • Sensors
    • Imaging
    • Nanotechnology
    • Scientific Computing
      • Big Data
      • HPC/Supercomputing
      • Informatics
      • Security
    • Semiconductors
  • R&D Market Pulse
  • R&D 100
    • Call for Nominations: The 2025 R&D 100 Awards
    • R&D 100 Awards Event
    • R&D 100 Submissions
    • Winner Archive
    • Explore the 2024 R&D 100 award winners and finalists
  • Resources
    • Research Reports
    • Digital Issues
    • R&D Index
    • Subscribe
    • Video
    • Webinars
  • Global Funding Forecast
  • Top Labs
  • Advertise
  • SUBSCRIBE