BioLegend Inc. has released over 50 Brilliant Violet 421 antibody conjugates for use in flow cytometry and microscopy. The company developed these reagents using Brilliant Violet 421, the first in a series of novel polymer-based fluorophores developed by Sirigen. It is excited by the 405 nm violet laser, and detected with the 450/50 nm filter typically used for Pacific Blue and BD Horizon V450. Brilliant Violet 421 antibodies provide brightness, stability, reliability, and improved signal-to-noise over Pacific Blue by 10-fold or more.
Brilliant Violet 421 will enable researchers to resolve populations in multi-color flow cytometry analysis, as the brightness of BV421 is on the same level as that of PE, according to the company,
Brilliant Violet 421 antibodies are compatible with currently used buffers and fixatives and common cell staining techniques, and are suitable for both surface and intracellular staining of antigens. The narrow excitation and emission spectra result in less requirement for cross-beam compensation and compensation into neighboring detectors.