Based on G-Biosciences’ Western ReProbe, this modified single component system is specifically formulated to dissociate and remove stubborn, high affinity antibodies from membrane-bound proteins without destroying the antigenic-binding affinity. Western ReProbe PLUS saves time and money by eliminating the need to re-run gels and transfer processes and by offering you the ability to re-use Western blots.
The Western ReProbe PLUS eliminates the need for boiling, denaturants, or SDS. The membrane-bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane-bound protein is free to accept new antibodies.
This system also allows screening of the same blot with different antibodies, repeated analysis and correction of unsatisfactory blots with multiple probes, and comparison of phosphorylated and total protein on the same blot. Each 100 mL supply is sufficient for up to 30 standard size Western blots.