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Artificial enzymes to reduce carbon dioxide emissions

By R&D Editors | May 19, 2015

Dr. Hernandez analyses the composition of water from a process for cleaning smoky carbon dioxide. Credit: Univ. of Nottingham Malaysia CampusEnzymes are biological catalysts that accelerate chemical reactions, such as the conversion of gaseous carbon dioxide into carbonates. Carbonates are the basic component of coral reefs, mollusc shells, geological platforms and kidney stones. Although naturally occurring enzymes would be ideal for converting human-generated carbon dioxide emissions into carbonates, they are generally incapable of coping with the extreme conditions of industrial plants.

Led by Dr. Ernesto Hernandez of the Univ. of Nottingham Malaysia Campus, researchers in Denmark, Malaysia, Spain and the U.K. are now developing artificial enzymes that can withstand the harsh environments of industrial plants while accelerating chemical reactions. His team ultimately aims to create a clean, cheap, practical and socially responsible solution for global warming by reducing carbon dioxide emissions.

“We believe that our novel artificial enzymes will be the first tailor-made enzymes for industrial plants to produce carbonates,” says Dr. Hernandez. So far, Dr. Hernandez and his colleagues have built an artificial environment composed of chimney-like equipment, measuring 1.5 m in height and 15 cm in diameter, that mimics the smoke released by power plants. Using the artificial environment, the researchers will ensure that their artificial enzymes can function properly under extreme conditions consisting of hot, corrosive, poisonous and sticky smoke as well as soot and other gases produced by power plants.

The team is basing the development of its artificial enzyme on naturally occurring carbonic anhydrase (CA), which accelerates the conversion of carbon dioxide into carbonates. Carbonic anhydrase is capable of turning carbon dioxide molecules into carbonates at a rate of one million molecules per second. However, “the enzyme’s carbon dioxide conversion rate slows down dramatically under industrial conditions,” Dr. Hernandez points out.

He and his colleagues are now engineering artificial enzymes based on natural CA, using directed evolution techniques. Their first step involves the creation of a library of diverse genes that encode for carbonic anhydrases. “This library includes sequences of unique forms of carbonic anhydrases recently found near deep-ocean chimneys (hydrothermal vents),” says Dr. Hernandez.

The team plans to modify and multiply the genes encoding for carbonic anhydrases using a molecular technique called random mutagenesis. The researchers will then place the mutated genes in the artificial environment to see which ones are most effective at converting carbon dioxide into carbonates. The best mutations will then be put through the modification and multiplication processes again. The researchers will repeat the whole process until they have isolated a mutated gene encoding for recombinant carbonic anhydrase that can convert carbon dioxide into carbonates under industrial conditions.

With the help of artificial enzymes, carbon dioxide-converted carbonates could be used in everything from baking soda and chalk to Portland cement and lime manufacturing.

Source: Univ. of Nottingham Malaysia Campus

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