Research & Development World

  • R&D World Home
  • Topics
    • Aerospace
    • Automotive
    • Biotech
    • Careers
    • Chemistry
    • Environment
    • Energy
    • Life Science
    • Material Science
    • R&D Management
    • Physics
  • Technology
    • 3D Printing
    • A.I./Robotics
    • Software
    • Battery Technology
    • Controlled Environments
      • Cleanrooms
      • Graphene
      • Lasers
      • Regulations/Standards
      • Sensors
    • Imaging
    • Nanotechnology
    • Scientific Computing
      • Big Data
      • HPC/Supercomputing
      • Informatics
      • Security
    • Semiconductors
  • R&D Market Pulse
  • R&D 100
    • Call for Nominations: The 2025 R&D 100 Awards
    • R&D 100 Awards Event
    • R&D 100 Submissions
    • Winner Archive
    • Explore the 2024 R&D 100 award winners and finalists
  • Resources
    • Research Reports
    • Digital Issues
    • R&D Index
    • Subscribe
    • Video
    • Webinars
  • Global Funding Forecast
  • Top Labs
  • Advertise
  • SUBSCRIBE

Cell Division more Complex than Previously Thought

By R&D Editors | May 7, 2012

Cell Division more Complex than Previously Thought

3-D model of rMTOC arrangement
In the wildtype cells, numbers of microtubules are emanating from the area just above the SPB
Scientists make Stunning Inner Space Observations
Top images: A series of fluorescent images of a spindle taken before the cut and at 5 seconds and 10 seconds after the cut. Scale bar, 10 micrometers. Bottom images: A graphical representation of the cut microtubules. The cut generates new ‘plus’ ends (red) and new ‘minus’ ends (green). The newly generated minus ends remain stable, whereas the new plus ends depolymerize, which creates two depolymerization fronts of opposed polarity. Courtesy of Jan Brugués

The mitotic spindle, an apparatus that segregates chromosomes during cell division, may be more complex than the standard textbook picture suggests, according to researchers at the Harvard School of Engineering and Applied Sciences (SEAS).

The findings, which result from quantitative measurements of the mitotic spindle, appeared April 27, 2012, in the journal Cell.

The researchers used a femtosecond laser to slice through the strands of the organelle and then performed a mathematical analysis to infer the microscopic structure of the spindle from its response to this damage.

“We’ve been using this nanosurgery technique to understand the architecture and assembly of the spindle in a way that was never possible before,” says Eric Mazur, Balkanski Professor of Physics and Applied Physics at Harvard, who co-authored the study. “It’s very exciting.”

The spindle, which is made of protein strands called microtubules, forms during cell division and segregates chromosomes into the daughter cells. It was previously unclear how microtubules are organized in the spindles of animal cells, and it was often assumed that the microtubules stretch along the length of the entire structure, pole-to-pole.

Mazur and his colleagues demonstrated that the microtubules can begin to form throughout the spindle. They also vary in length, with the shortest ones close to the poles.

“We wondered whether this size difference might result from a gradient of microtubule stabilization across the spindle, but it actually results from transport,” says lead author Jan Brugués, a postdoctoral fellow at SEAS. “The microtubules generally nucleate and grow from the center of the spindle, from which point they are transported towards the poles. They disassemble over the course of their lifespan, resulting in long, young microtubules close to the midline and older, short microtubules closer to the poles.”

“This research provides concrete evidence for something that we’ve only been able to estimate until now,” Brugués adds.

Mazur and Brugués worked with principal investigator Daniel Needleman, Assistant Professor of Applied Physics and Molecular and Cellular Biology at Harvard, and Valeria Nuzzo, a former postdoctoral fellow in Mazur’s lab at SEAS, to bring the tools of applied physics to bear on a biological question.

The team used a femtosecond laser to make two small slices perpendicular to the plane of growth of the spindle apparatus in egg extracts of the frog species Xenopus laevis.

They were then able to collect quantitative data on the reconstruction of the spindle following this disruption and precisely determine the length and polarity of individual microtubules. Observing the speed and extent of depolymerization (unraveling) of the spindle, the team worked backward to compile a complete picture of the beginning and end points of each microtubule. Finally, additional experiments and a numerical model confirmed the role of transport.

“The laser allowed us to make precise cuts and perform experiments that simply were not possible using previous techniques,” says Mazur.

With further inquiries into spindle architecture, the researchers hope that scientists will one day have a complete understanding, and possibly even control over, the formation of the spindle.

“Understanding the spindle means understanding cell division,” notes Brugués. “With a better understanding of how the spindle is supposed to operate, we have more hope of tackling the range of conditions — from cancer to birth defects — that result from disruptions to the cell cycle or from improper chromosomal segregation.”

The research was supported by the National Science Foundation and by a fellowship from the Human Frontiers Science Program.

Video 1: http://www.seas.harvard.edu/news-events/images/Mitotic%20spindle%20cut.mov

A pulse from the femtosecond laser slices through the mitotic spindle, causing the damaged microtubules to depolymerize. Meanwhile, undamaged and newly nucleated microtubules continue growing and soon fill the gap. Courtesy of Jan Brugués

Video 2: http://www.seas.harvard.edu/news-events/images/Mitotic%20spindle%20formation.mov

To conduct the experiments, the research team induced spindle formation in Xenopus egg extracts. This video shows the initial formation of the spindle. Courtesy of Jan Brugués

Related Articles Read More >

QED-C outlines road map for merging quantum and AI
Quantum computing hardware advance slashes superinductor capacitance >60%, cutting substrate loss
Hold your exaflops! Why comparing AI clusters to supercomputers is bananas
Why IBM predicts quantum advantage within two years
rd newsletter
EXPAND YOUR KNOWLEDGE AND STAY CONNECTED
Get the latest info on technologies, trends, and strategies in Research & Development.
RD 25 Power Index

R&D World Digital Issues

Fall 2024 issue

Browse the most current issue of R&D World and back issues in an easy to use high quality format. Clip, share and download with the leading R&D magazine today.

Research & Development World
  • Subscribe to R&D World Magazine
  • Enews Sign Up
  • Contact Us
  • About Us
  • Drug Discovery & Development
  • Pharmaceutical Processing
  • Global Funding Forecast

Copyright © 2025 WTWH Media LLC. All Rights Reserved. The material on this site may not be reproduced, distributed, transmitted, cached or otherwise used, except with the prior written permission of WTWH Media
Privacy Policy | Advertising | About Us

Search R&D World

  • R&D World Home
  • Topics
    • Aerospace
    • Automotive
    • Biotech
    • Careers
    • Chemistry
    • Environment
    • Energy
    • Life Science
    • Material Science
    • R&D Management
    • Physics
  • Technology
    • 3D Printing
    • A.I./Robotics
    • Software
    • Battery Technology
    • Controlled Environments
      • Cleanrooms
      • Graphene
      • Lasers
      • Regulations/Standards
      • Sensors
    • Imaging
    • Nanotechnology
    • Scientific Computing
      • Big Data
      • HPC/Supercomputing
      • Informatics
      • Security
    • Semiconductors
  • R&D Market Pulse
  • R&D 100
    • Call for Nominations: The 2025 R&D 100 Awards
    • R&D 100 Awards Event
    • R&D 100 Submissions
    • Winner Archive
    • Explore the 2024 R&D 100 award winners and finalists
  • Resources
    • Research Reports
    • Digital Issues
    • R&D Index
    • Subscribe
    • Video
    • Webinars
  • Global Funding Forecast
  • Top Labs
  • Advertise
  • SUBSCRIBE