Enzo Life Sciences expanded its offering of Cellestial Cellular Analysis products with the introduction of the Cyto-ID Red Long-Term Cell Tracer Kit. Using a proprietary non-covalent cell labeling technology, the Cyto-ID Red Long-Term Cell Tracer Kit provides a method for fluorescent labeling of live cells over an extended period of time. The dye is well retained by cells for up to 96 hours after loading, and is passed to daughter cells upon mitosis with decreased fluorescence intensity.
The red fluorescent dye incorporates into the cell’s plasma membrane with no apparent toxic effect, according to the company. Since the dye does not covalently modify proteins within the cells, normal physiological responses are well preserved. Cyto-ID Red Tracer Dye fluorescence is independent of pH within normally encountered physiologic ranges and fluorescence intensity per cell is typically unaffected by the ultimate pattern of dye distribution.
The kit is suitable for tracing cell lineages, as well as assaying proliferation, precursor frequency, chemotaxis, migration, phagocytosis, and cell- and antibody-mediated cytotoxicity. Analysis of labeled and unlabeled cell populations over time by flow cytometry or microscopy has been validated.
