EMD Millipore introduced the Magna ChIP HT96 kit for high-throughput chromatin immunoprecipitation (ChIP). Using the kit, ChIP can be performed on up to 96 samples in a single experiment using as few as 10,000 cells per well. The optimized protocol uses a single, proprietary buffer for sonication, immunoprecipitation and washing which yields excellent sensitivity and lower backgrounds than conventional ChIP methods.
The Magna ChIP HT96 protocol has been designed and optimized for efficient immunoprecipitation and recovery of DNA. Consequently, in many cases, a separate DNA purification step is not required for downstream analysis.
ChIP is used for mapping the in vivo distribution of proteins associated with chromosomal DNA. For many laboratories, ChIP can be technically demanding, requiring robust antibodies, high quality reagents, and careful planning and handling. The Magna ChIP HT96 kit was designed to address these challenges when processing a larger number of samples.
The Magna ChIP HT96 kits utilize a magnetic protein A/G bead blend that provides multiple advantages over single-bead approaches. In contrast to kits with either protein A or protein G, the Magna ChIP A/G beads allow affinity capture of a broader range of antibody isotypes. In addition, the magnetic properties of these beads permit rapid processing of ChIP samples to maintain high fold enrichment when used with multichannel pipettes and magnetic separation stand or with the use of an automated liquid handler.
The kit is available with or without control antibodies and qPCR analysis primers.
EMD Millipore offers a wide selection of highly specific antibodies demonstrated to work in ChIP (ChIP-qualified antibodies) as well as a collection of rigorously ChIP-validated antibodies and control primer sets known as ChIPAb+ kits.
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