The DeskGen Platform for gene editing combines elements of AutoClone and gUIDEbook and allows researchers to design genome editing vectors and cloning strategies optimized to their own laboratories and experimental needs. It provides the power to expertly design optimized genome editing experiments, in any cell line and species, right from the desktop. The platform has two modules: one for experimental design, and a second to manage DNA inventories, parts lists and feature annotations. The experimental module improves upon the best elements of publicly-available CRISPR tools through the use of laboratory validation, overlaying the results in an information-rich genome browser and building more robust algorithms that provide users with a deeper level of analysis. The repository module manages users’ DNA inventories, feature annotations and sequence information.