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Genomic Blocks

By R&D Editors | January 31, 2012

Integrated DNA Technologies (IDT), a leader in oligonucleotide synthesis, has launced a significant advancement in synthetic biology tools. gBlocks Gene Fragments are double-stranded, sequence-verified genomic blocks up to 500 base pairs. Their high sequence fidelity and rapid delivery time makes gBlocks Gene Fragments ideal for a range of biology applications, including easy assembly of multiple gene fragments to reliably generate larger gene constructs.

gBlocks Gene Fragments significantly reduce the cost for synthetic gene synthesis to less than US$0.20 per base pair. Cost for traditional gene synthesis currently exceeds US$0.30 per base pair. gBlocks Gene Fragments are provided as linear double-stranded DNA rather than already cloned into a vector, meaning that they can be easily and quickly utilized for a wide range of applications including custom protein synthesis, microRNA analysis, in vitro transcription and many more. For this reason, they are available with or without 5’ phosphate modification depending on the required application.

Each order is supplied as 200ng of dried DNA, ensuring maximal stability prior to use. Although the blocks are versatile enough to use with most published synthesis methods such as cloning with blunt ends, restriction sites or T/A overhangs, when combined via isothermal gene assembly, it is possible to create a completely custom 2kb construct in less than an hour. Combined, these features allow scientists to speed up their research and increase the number of gene design variants they include in their experiments.

Integrated DNA Technologies

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