QIAGEN offers the Type-it HRM PCR Kit and Rotor-Gene ScreenClust HRM Software, HRM (high resolution melting) technology enabling fast, accurate genotyping results.
Genotyping is widely used to study genetic disorders such as human disease or cancer. Researchers rely on HRM to quickly and accurately detect and characterize known or previously unknown sequence variations down to a single base pair difference. HRM is an easier, more cost-effective method than traditional genotyping analysis, according to QIAGEN.
The new Type-it HRM PCR kit enables fast, accurate detection of gene mutations and SNPs. The Type-it HRM PCR Kit is well-suited for all genotyping research applications including typing of disease or cancer loci, biomarker discovery and pathogen detection. The Type-it HRM PCR Kit does not require optimization in the development of new HRM assays, thus delivering consistent performance the first time.
The Type-it HRM PCR Kit combined with the Rotor-Gene Q Real-time PCR and HRM Instrument delivers outstanding results. The rotary design of the Rotor-Gene Q combined with its thermal and optical performance makes it a highly suitable instrument for HRM. Minute differences in melting profiles such as the most difficult Class IV SNPs can be easily interpreted.
The Rotor-Gene ScreenClust HRM Software from QIAGEN overcomes the constraints of other software solutions by offering a stringent statistical approach to extract, process and group sample characteristics in HRM data sets automatically. This approach even allows genotyping without control samples. In combination with the Rotor-Gene Q or Rotor-Gene 6000, researchers are able to faster and more reliably differentiate between alleles.