G-Biosciences offers a unique product for the purification of protein from bacterial samples. Our HOOK GST Protein Purification kits allow for the rapid extraction of soluble, GST tagged protein. The bacteria are first lysed with supplied Bacterial PE LB and PE LB-Lysozyme to release total soluble protein while maintaining the structure and activity of the protein.
The GST tagged protein is purified by affinity chromatography and the resin is transferred to a convenient spin column where it is rapidly washed and the GST protein is eluted.
Available in a gravity flow format for yields of 10mg/250ml of culture and spin kits for rapid elution of tagged protein for ~1mg/50ml of culture.
Also available are G-Biosciences’ HOOK GST Protein Purification kits for yeast.