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Luciferase

By R&D Editors | June 1, 2012

The NanoLuc Luciferase from Promega Corp. includes a novel substrate, furimazine, and a enzyme developed using directed evolution techniques. It has small size, allowing for enhanced viral delivery and protein fusion applications, and it is easily secreted from cells.


The enzyme is two orders of magnitude brighter than either firefly (Photinus pyralis) or Renilla reniformis luciferases resulting in better performance in hard to transfect cells. NanoLuc performs in more physiologically relevant models including complex biological samples. With add and measure simplicity and a stable signal, NanoLuc Luciferase can be scaled from bench-top to high-throughput screening applications with no modifications.


To meet the many application needs in a ‘next-gen’ genetic reporter, 12 plasmid versions of NanoLuc Luciferase are available. The offerings include NanoLuc-PEST (NlucP), which closely couples protein expression to changes in transcriptional activity and increased signal-to-background ratios. For a secreted reporter, a NanoLuc Luciferase construct has been prepared by fusion to an N-terminal secretion signal (secNluc). Using the Nano-Glo Luciferase Assay Reagent, luminescence is linear over a 1,000,000-fold concentration range with a signal half-life  of 2 hours.


Promega Corp.

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