2D electrophoresis and mass spectrometry is routinely used for identification of novel proteins. However, the greatest challenge in protein identification is achieving suitable resolution of proteins. The high dynamic range of a species’ proteome means that the more abundant proteins mask the less abundant and often more interesting proteins.
By fractionation, one simplifies a protein mixture by reducing the amount and the number of protein species to be loaded onto the gel matrix. Fractionation produces less crowded individual protein maps, simplifying analysis and interpretation.
G-Biosciences has developed a simple and reproducible method of serial fractionation of total cellular proteins. Fraction-FOCUS utilizes proven technology to fractionate and concentrate all proteomes into multiple fractions. The kit simplifies the protein composition and allows for improved resolution and simplified 2D maps, which in turn allows for improved analysis and interpretation and greatly increases the chances of identifying novel and less abundant proteins. G-Biosciences’ Fraction-FOCUS is fully compatible with 2D electrophoresis or isoelectric focusing and other applications.