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Protein Identification

By R&D Editors | July 14, 2009

lucidIDBio-Rad Laboratories, Inc., a multinational manufacturer and distributor of life science research and clinical diagnostic products, launched the Lucid ID Access Pack, the first product designed for the Lucid Proteomics System, a proteomics solution in development by Bio?Rad and Bruker Corporation that will combine Bio-Rad’s ProteinChip surface-enhanced laser desorption/ionization (SELDI)-based array technology with Bruker’s ultrafleXtreme MALDI-TOF/TOF mass spectrometer, enabling researchers to use both top-down and bottom-up proteomics approaches for biomarker discovery on the same system. With the Lucid ID Access Pack, researchers who currently profile their biomarkers using Bio?Rad’s ProteinChip array technology can use the Lucid ID Access Pack to complete the biomarker purification process and identify these biomarkers with high confidence.

A distinct advantage of the Lucid ID Access Pack is the ability to identify peptides under 4 kilodaltons directly on a ProteinChip array after a simple, one-step enrichment. Identification of proteins and peptides over 4 kilodaltons that require trypsin digestion is demystified by following Bio?Rad’s Biomarker Purification and Identification Guide and by using ProteinChip arrays in conjunction with Bruker’s ultrafleXtreme MALDI-TOF/TOF, which has been optimized for the ability to read ProteinChip arrays. This new high-performance system delivers the resolution required for rapid, high-confidence protein identification. For convenience, the Lucid ID Access Pack is also backwards-compatible with other Bruker autofleX and ultrafleX MALDI TOF/TOF systems.

The Lucid ID Access Pack contains the essential accessories and consumables needed for high-confidence identification of putative biomarkers, including:

· A chip holder specifically designed to analyze ProteinChip arrays on the Lucid Proteomics System
· ProteinChip arrays with chromatographic surfaces most commonly used in identification protocols
· A manual including optimized instrument settings for on-chip peptide identification
· All necessary user licenses
· Personalized consulting with premier experts on purification and enrichment strategies

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