GE Healthcare’s HiTrap TALON crude, His SpinTrap TALON microspin, His MultiTrap TALON and His GraviTrap TALON are pre-packed with TALON Superflow media for rapid screening, preparative purification and easy scale-up of histidine-tagged proteins. Samples derived from a variety of expression systems can be readily purified with minimal sample preparation time and no additional equipment costs.
TALON Superflow is a cobalt-based medium designed for the purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Compared to nickel-charged media, cobalt-based media offers enhanced selectivity and yield purity for histidine-tagged proteins. Additionally, lower concentrations of imidazole are required during purification which better preserves the structure and function of sensitive histidine-tagged proteins.
Key features of the TALON Superflow medium and pre-packed formats:
• Suitable for IMAC purification of histidine-tagged proteins when Ni2+ is not the optimal choice of metal ion
• High yield purity in a single step using a mild purification process which preserves structure and function of sensitive histidine-tagged proteins
• Prepacked HiTrap TALON crude 1 ml and 5 ml columns and His GraviTrap TALON gravity flow columns allow direct loading of unclarified samples to minimize labor and protein degradation
• His SpinTrap TALON spin columns and His MultiTrap TALON 96-well plates enable screening with highly reproducible column-to-column and well-to-well results