EMD Millipore’s Direct Detect system offers fast, accurate protein quantitation with minimal sample preparation. The infrared (IR)-based spectrometry system and novel membrane technology accurately quantifies amide bonds intrinsic to all proteins, distinguishing proteins from interfering sample components. Because IR-based quantitation does not rely on amino acid composition, dye binding, or redox potential, this approach surpasses conventional UV-Vis spectroscopy and colorimetric assays and allows protein quantification in the presence of reducing agents and detergents.
The system requires only a small sample volume (2 µL) and can measure protein concentrations from 0.2 mg/mL to 5 mg/mL within minutes. In addition, the system’s calibration standard curve needs to be generated only once, providing additional time savings and ease of use. The Direct Detect system is also capable of quantifying other biomolecules, including lipids and nucleic acids.
