DNA2.0 introduced a complete line of Protein Variant Libraries designed to help researchers better engineer proteins. Four key library options—alanine scanning, single site saturation, combinatorial site, and antibody—are suited for the most common methods of protein engineering, although DNA2.0 can produce additional libraries, such as Modular, Truncation or Random Mutagenesis Libraries, depending on research needs. Libraries are delivered ready for screening to identify variants with improved or altered function.
The emerging field of protein engineering offers an extraordinary opportunity to design novel proteins that can be utilized for a wide range of purposes such as creating antibody-based therapeutics or specialized enzymes for energy production or to remove toxins from the environment. By utilizing libraries to screen for desired activity through directed evolution, researchers can more quickly and efficiently engineer useful proteins. DNA2.0’s sequence libraries are powerful tools for structure-function relationship analysis or for modifying the activities of proteins or DNA regulatory regions.
“DNA2.0’s Protein Variant Libraries are tailored to your particular research question, whether you want to improve your protein yield with a Combinatorial Site Library or increase the affinity or specificity of an antibody with an Antibody Library,” said Claes Gustafsson, COO and cofounder of DNA2.0. “While DNA2.0‘s ProteinGPS system for rational protein design is the preferred approach to protein engineering when screening capacity is limited, we are pleased to offer the flexibility and affordability of our Protein Variant Libraries for our customers who are best served by a directed evolution approach.”
Each of DNA2.0’s core family of Protein Variant Libraries is designed to tackle a specific research question:
• Alanine Scanning Libraries can determine which amino acid residues are critical for protein function. Each alanine substitution allows examination of the contribution of an individual amino acid sidechain to the functionality of the protein.
• Single Site Saturation Libraries identify advantageous substitutions at a specific single site within a protein for enhanced protein properties.
• Combinatorial Site Libraries test multiple positions with a smaller amount of variation at each position. Typically, 2-12 positions with 2-5 substitutions at each position are investigated. This library is best used to screen a small region of a protein, or to follow up on improved amino acid substitutions identified in a Single Site Saturation Library.
• Antibody Libraries introduce complete or tailored degeneracy into CDRs of one or more genes to increase the affinity and specificity of an antibody.
DNA2.0’s library technologies platform has been successfully applied by Adimab, Inc (Lebanon, NH) to produce proprietary libraries that replicate the human immune system. Adimab is then able to move from antigen to purified, full-length human IgGs quickly.
In addition to the primary libraries, DNA2.0 offers Modular Libraries to allow the building of new genetic modules or pathways from basic DNA parts, Random Mutagenesis Libraries to highlight changes in function with a high degree of variability and a Truncation Library to identify protein domain borders or functional protein size requirements.
DNA2.0’s Library Design Specialists can help determine the best library or series of libraries to suit the needs of a particular project or research goal. DNA2.0 Protein Variant Libraries are customized for individual research needs and are delivered with the same industry-leading speed and customer service that DNA2.0 guarantees for its complete line of bioengineering solutions.