Research & Development World

  • Home Page
  • Topics
    • Aerospace
    • Archeology
    • Automotive
    • Biotech
    • Chemistry
    • COVID-19
    • Environment
    • Energy
    • Life Science
    • Material Science
    • R&D Market Pulse
    • R&D Management
    • Physics
  • Technology
    • 3D Printing
    • A.I./Robotics
    • Battery Technology
    • Controlled Environments
      • Cleanrooms
      • Graphene
      • Lasers
      • Regulations/Standards
      • Sensors
    • Imaging
    • Nanotechnology
    • Scientific Computing
      • Big Data
      • HPC/Supercomputing
      • Informatics
      • Security
      • Software
    • Semiconductors
  • 2021 R&D 100 Award Winners
    • R&D 100 Awards
    • 2020 Winners
    • Winner Archive
  • Resources
    • Digital Issues
    • Podcasts
    • Subscribe
  • Global Funding Forecast
  • Webinars

Researchers unveil molecular machine that initiates DNA transcription

By R&D Editors | October 19, 2012

A team of Rutgers University scientists led by Richard H. Ebright and Eddy Arnold has determined the three-dimensional structure of the transcription initiation complex, the key intermediate in the process by which cells read out genetic information in DNA.

In a paper to be published in Science and released online today at Science Express, the Rutgers scientists show how the “molecular machine” responsible for transcription initiation—a protein complex that consists of the enzyme RNA polymerase and the initiation factor sigma—recognizes a specific site on DNA preceding a gene, binds to DNA, unwinds the DNA helix, and pre-organizes the unwound DNA to enable subsequent reactions.

“Determining the structure of a functional, specific transcription initiation complex has been a goal of researchers for three decades,” said Ebright, a professor in the Department of Chemistry and Chemical Biology at Rutgers, a laboratory director at the Waksman Institute of Microbiology at Rutgers, and an investigator of the Howard Hughes Medical Institute.

The structure determined by the Rutgers researchers is the structure of a transcription initiation complex from a bacterium. The structure provides a foundation for understanding bacterial transcription initiation and transcriptional regulation and provides a starting point for developing new antibacterial agents that function by inhibiting bacterial transcription. Because the transcription machineries in bacteria and higher organisms are structurally and mechanistically related, the structure also provides a framework for understanding transcription and transcriptional regulation in higher organisms, including humans.

The structure defines the interactions that RNA polymerase and sigma make with the DNA site for transcription initiation, known as the “promoter.” In particular, the structure defines interactions with a segment of the promoter that RNA polymerase and sigma unwind to form single-stranded DNA (the “transcription bubble”) and specific DNA sequences that RNA polymerase and sigma recognize and bind to within this segment of the promoter (the “-10 element,” the “discriminator element,” and a new DNA sequence identified in this work, the “core recognition element”).

The structure shows that a first part of sigma recognizes the -10 element through contacts with single-stranded DNA that entail the unstacking and insertion of DNA bases of the -10 element into pockets. A second part of sigma recognizes the discriminator element through contacts with single-stranded DNA that entail the unstacking and insertion of a DNA base of the discriminator element into a pocket. A third part of sigma contacts the other strand of DNA and pre-organizes it to serve as the template for RNA synthesis. Finally, RNA polymerase recognizes the core recognition element through contacts with single-stranded DNA, unstacking and inserting a DNA base into a pocket.

“This study represents a very significant contribution to our understanding of the workings of this central macromolecular machine of gene expression,” said Peter von Hippel, professor of biophysical chemistry and molecular biology at the University of Oregon, who was not part of the study. “A particular significance of this work is the very systematic way the researchers built nucleic acid scaffolds bound to various nucleic acid and protein complexes involved in the various steps of initiation and were able to show in detail how the sigma initiation factor interacts with the various individual nucleotide residues involved in the recognition of the important elements of the promoter.”

Structural Basis of Transcription Initiation

Source: Rutgers University

Related Articles Read More >

Novel mass spectrometry solution simplifies insight gathering into macromolecular complexes
ENPICOM launches display solution to accelerate antibody selection while maximizing precision
Thermo Fisher Scientific autoimmune-testing instruments now available in the U.S.
Thermo Fisher Scientific and Qatar Genome Program partner to advance precision medicine 
2021 R&D Global Funding Forecast

Need R&D World news in a minute?

We Deliver!
R&D World Enewsletters get you caught up on all the mission critical news you need in research and development. Sign up today.
Enews Signup

R&D World Digital Issues

February 2020 issue

Browse the most current issue of R&D World and back issues in an easy to use high quality format. Clip, share and download with the leading R& magazine today.

Research & Development World
  • Subscribe to R&D World Magazine
  • Enews Sign Up
  • Contact Us
  • About Us
  • Drug Discovery & Development
  • Pharmaceutical Processing
  • 2021 Global Funding Forecast

Copyright © 2022 WTWH Media LLC. All Rights Reserved. The material on this site may not be reproduced, distributed, transmitted, cached or otherwise used, except with the prior written permission of WTWH Media
Privacy Policy | Advertising | About Us

Search R&D World

  • Home Page
  • Topics
    • Aerospace
    • Archeology
    • Automotive
    • Biotech
    • Chemistry
    • COVID-19
    • Environment
    • Energy
    • Life Science
    • Material Science
    • R&D Market Pulse
    • R&D Management
    • Physics
  • Technology
    • 3D Printing
    • A.I./Robotics
    • Battery Technology
    • Controlled Environments
      • Cleanrooms
      • Graphene
      • Lasers
      • Regulations/Standards
      • Sensors
    • Imaging
    • Nanotechnology
    • Scientific Computing
      • Big Data
      • HPC/Supercomputing
      • Informatics
      • Security
      • Software
    • Semiconductors
  • 2021 R&D 100 Award Winners
    • R&D 100 Awards
    • 2020 Winners
    • Winner Archive
  • Resources
    • Digital Issues
    • Podcasts
    • Subscribe
  • Global Funding Forecast
  • Webinars