The discovery of myriad roles for small RNAs in gene expression has increased demand for products capable of isolating them in high purity and quantity. With the success of all downstream applications dependent upon sample integrity, selection of the RNA isolation method is critical. AMRESCO offers RNA isolation and purification reagents to suit diverse research objectives, including phenol-based RiboZol RNA Extraction Reagent, RiboZol Plus RNA Purification Kit, and a Phenol-Free Total RNA Purification Kit. Now AMRESCO offers an additional choice, RiboZol ME (Micro Enriched), a variation of the widely popular RiboZol RNA Extraction Reagent.
Like its predecessor, RiboZol ME follows a simple protocol whereby a variety of cell and tissue types can be homogenized directly in the single phase phenol solution, minimizing degradation of RNA by RNases. In contrast to RiboZol RNA Extraction Reagent, RiboZol ME is specially formulated to enrich the RNA sample with small molecular weight RNA while reducing the concentration of large molecular weight RNA. Although the sample ultimately contains both large and small RNA, they are present in a ratio that is ideal for miRNA discovery without sacrificing the ability to simultaneously analyze large molecular weight RNA.
RiboZol ME is an excellent alternative to standard phenol-based and column-based extraction methods for small RNA. For an affordable price, the RiboZol ME yields high quality total RNA enriched with small molecular weight RNA. The isolation protocol is simple, completely scalable and requires no additional steps or time compared to typical total RNA extraction with organic reagent. RiboZol ME works with most tissues and the isolated RNA sample is compatible with all common downstream RNA applications, such as qPCR and Northern blotting.