Sentient Suite is designed for Life Science and Translational Research. It integrates data, applications, databases and instruments into one secure and compliant interoperable environment. Users can access, query and use data from multiple sources regardless of format and location, using existing hardware and IT infrastructure. Administrators and end-users can define data-driven informatics applications without programming. The suite includes Data Manager, Image Interactor, Web Query, Knowledge Explorer and Process Manager. Knowledge Explorer leverages the latest semantic web technologies to integrate, model, visualize and explore data, revealing networks and novel relationships in complex data sets from one or multiple sources, while Data Manager facilitates user-driven and automated data import and structuring. Process Manager is a Web-based drag-and-drop configurable workflow engine. Web Query allows Web-access through a browser to any relational database and the Sentient data store, and Image Interactor provides analytical and descriptive image interaction for virtually any type of image.