G-Biosciences’ 1-Step RT-PCR kit is designed for optimal convenience in carrying out highly sensitive and specific reverse transcription polymerase chain reactions (RT-PCR) in a single tube. 1-Step RT-PCR is a variation of the standard two-step RT-PCR, in which all components of the RT and PCR are mixed in one tube prior to starting the reactions so that RT and PCR can be carried out sequentially in one tube.
G-Biosciences’ one step method offers tremendous convenience when applied to analysis of single targets from multiple RNA samples. This approach also minimizes the possibility for introduction of contaminants into reactions between the RT and PCR steps, since the RT and PCR reactions are carried out uninterruptedly without opening the reaction tubes in between steps.
G-Biosciences’ 1-Step RT-PCR Kit is composed of high quality reagents. The AMV Reverse Transcriptase (from Avian Myeloblastosis Virus) is used for RT, and the optimized enzyme Taq DNA Polymerase (from Thermus aquaticus) is used for PCR. Our kit also includes a unique 10X concentrated RT-PCR buffer that is suitable for both the RT and PCR steps and is crucial for successful 1-Step RT-PCR, our dNTP mixture, ribonuclease inhibitor and DEPC-treated water.