Spectronaut is softwarefor analysis of hyper reaction monitoring, HRM-MS (SWATH, DIA) data. HRM-MS is a targeted proteomics technology based on data-independent acquisition (DIA) performed on new generation mass spectrometric systems. Spectral library generation capability based on MaxQuant search results is incorporated into the software. It supports spectral library generation from the Thermo Scientific Proteome Discoverer platform, as well as the analysis of DIA data generated on the Thermo Scientific Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer. HRM enables reproducible and accurate quantification of 1000s of proteins in a single instrument run. Software algorithms are integrated with a reagent kit — HRM Calibration Kit — that provides customized parameter calibration and quality control.