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Toward a faster, more accurate way to diagnose stroke

By R&D Editors | May 7, 2014

A much-needed, rapid test for stroke—caused by the sudden stop of blood flow in the brain—is on the horizon. Credit: lixuyao/iStock/Thinkstock When someone suffers from a stroke, a silent countdown begins. A fast diagnosis and treatment can mean the difference between life and death. So scientists are working on a new blood test that one day could rapidly confirm whether someone is having a stroke and what kind. Their report appears in the American Chemical Society journal Analytical Chemistry.

Steven A. Soper and colleagues note that strokes, which are the third leading cause of death and disability in the United States, have two possible causes. In ischemic strokes, a clot stops blood flow in a part of the brain. In hemorrhagic stroke, a blood vessel in the brain ruptures. Both can lead to similar symptoms, such as numbness on one side of the body, sudden weakness and headache. Current diagnostic tests can’t tell between these two types. But treatment, ideally within three hours of onset, depends on the kind of stroke a person is having. Soper’s collaborator, Alison Baird, who is at SUNY Downstate Stroke Center, found clues—or biomarkers—in the blood that can suggest the stroke type and assist in determining the course of proper treatment. Soper’s team sought a way to detect those clues quickly.

They built a device that can process whole blood and isolate genetic material for two potential stroke biomarkers within minutes. Keeping in mind that identifying more biomarkers could aid in diagnosis, they designed their device so it can analyze a total of four biomarkers at the same time.

The authors acknowledge funding from the National Institute of Biomedical Imaging and Bioengineering.

Parallel Affinity-Based Isolation of Leukocyte Subsets Using Microfluidics: Application for Stroke Diagnosis

Source: American Chemical Society

 

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