Facilitating Analysis and Detection for SFC

Software integration enables hyphenated SFC analysis

The pace of analysis and purification labs is increasing at a dizzying clip. Methods are to become shorter, column robustness is taxed by the increasing flows and

pressures of HPLC systems, and hyphenated detector identification of peaks is just as important as relative purity. Supercritical fluid chromatography is an alternative chromatographic technique that has been adopted by many in the pharmaceutical industry to alleviate some of the previously stated burdens. SFC manufacturers have toiled with marketing a robust and reliable platform that includes control of the system, detectors, and fraction collectors, in a seamless and universally accepted software package. The following article will demonstrate the possibilities with the full integration of SFC into MassLynx and FractionLynx software from Waters Corporation.

As the synthetic route to create active pharmaceutical ingredients (APIs) increases in complexity, the analysis of the reaction mixture becomes more difficult. The implementation of chromatographic techniques was adopted by the pharmaceutical industry to solve this analysis problem. High performance liquid chromatography (HPLC), due to its great efficiency, has become the standard of analysis. The hyphenated technique enables the user to identify the chemical entities in a matrix and determine relative abundance of desired product or impurity when combined with light absorption data. Now an analyst can gather a similarly significant amount of high-resolution data using SFC…

This article originally appeared in the August 2007 issue of Scientific Computing‘s Chromatography Techniques supplement. Click here to view the full article in PDF format.