HPLC is an analytical procedure for separation, identification and quantification of substances using liquid chromatography. In HPLC, the eluant must have especially high physical and chemical purity and may not contain suspended mechanical particles or any dissolved substances that can be released by the column at a delayed time and thus generate a signal. The quality of a solvent is often decisive for the reliability of an HPLC analytical run. In this study, we evaluate an affordable alternative to commercially sold ultrapure water to prepare high-purity eluants for HPLC analysis.
