The GenomeStack Big Data Analytics platform has been developed specifically for bioinformatics researchers, data scientists and analysts conducting genome research. The database replaces the traditional file-based, manual process for storing and analyzing genome sequenced data. Post-aligned sequencing data from multiple samples is stored in an ultra-fast and highly scalable SQL database, enabling querying of a large number of samples simultaneously. Thus, an analysis of 50 BAM files with 30 billion rows of aligned sequencing data, can be analyzed in under a minute, while providing a detailed drill-down from all participating samples to the single read level in order to review the nucleotides distribution on a specific chromosome position.