ZEISS has recently introduced two new software functionalities with ZEISS LSM Plus and ZEISS Airyscan Joint Deconvolution, users achieve better results in confocal microscopy.
Maximizing recovery requires improvements in efficiency
Laser scanning microscopes (LSMs) are the multifunctional high-end microscopes in every imaging facility, valued as much for their instant, high-quality imaging of optical sections as for their ease of use and application flexibility. ZEISS LSMs provide a wide range of detection modes with highest sensitivity and spectral flexibility for multi-fluorescent experiments. ZEISS LSM Plus and ZEISS Airyscan Joint Deconvolution significantly improve the data quality in confocal imaging. This enhancement is independent of the chosen detector, imaging mode and emission range.
ZEISS LSM Plus: Improving the whole confocal experience
The linear Wiener filter deconvolution of the new ZEISS LSM Plus option is based on the time-tested super-resolution processing of ZEISS Airyscan. It can be applied to data of any confocal imaging mode – including spectral, near infrared and multiphoton microscopy – with next to no interaction, ensuring reliable quantitative results. Users benefit from an enhanced signal-to-noise ratio at high acquisition speed and low laser power – particularly useful for live cell imaging of delicate samples. For samples that are not too light sensitive and therefore allow the pinhole of the LSM to be closed, more spatial information and even greater resolution enhancement can be obtained.
ZEISS Airyscan Joint Deconvolution: 32 views mean more information
ZEISS Airyscan 2 is an area detector with 32 circularly arranged detection elements. Each of these acts as a small pinhole with a slightly different view on the sample, providing additional spatial information. With the introduction of ZEISS Airyscan Joint Deconvolution, this additional spatial information can now be leveraged to reduce the distance that can be resolved between two points down to 90 nm. Super-resolution experiments benefit from an improved separation of single or multiple labels. Users immediately recognize the significance for their research: “When we imaged endoplasmic reticulum and mitochondria and saw their very fine details after applying ZEISS Airyscan Joint Deconvolution, we thought this is just super cool. The new option could be integrated within our imaging protocols very easily. We were amazed at how quickly the images were processed, which helped us make decisions while we were still imaging”, said Kelly Subramanian, PhD, post-doctoral scholar, Department of Molecular and Cellular Biology at UC Davis.
Users benefit from ease of use
These two new options enable users to reliably gather more information for all confocal experiments while increasing productivity. Particularly, imaging facilities with multiple users benefit from seamlessly integrated, easy-to-apply improvements – without any expert knowledge or extra effort. The existing ZEISS LSM 900, including ZEISS Celldiscoverer 7 and ZEISS LSM 980 microscope systems can be upgraded accordingly.
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