AMSBIO announced the Iso-Gold Rapid Mouse Monoclonal isotyping kits, a rapid-format isotyping kit that reduces the labor of the traditional ELISA protocol from a day-and-a-half procedure to a five-minute assay.
The Iso-Gold kit is a rapid lateral flow assay, with leading edge sensitivity (10ng/ml), that can be run on both tissue culture supernatant, as well as mouse ascites fluid.
In a single step, the diluted antibody sample is added into the loading well of the cassette. In less than five minutes, the Iso-Gold kit provides a color readout of the mouse monoclonal antibody isotope (IgG1, IgG2a, IgG2b, IgG3, IgA, or IgM). The test is also available with kappa and lambda light chains determination.
Iso-Gold kits are proven stable at room temperature for more than one year. The assay does not cross react with fetal bovine serum (FBS).
Published in Drug Discovery & Development magazine: Vol. 12, No. 5, May, 2009, p.35.