Thermo Fisher Scientific Inc.’s Nunclon Vita stem cell culture surface is an energy-treated polystyrene surface that enables direct attachment, colony formation, and growth of human embryonic stem cells (ESC) and induced pluripotent stem cells (IPS), without feeder cells or matrix coatings.
In media supplemented with a Rho-associated kinase (ROCK) inhibitor, human ESCs can be cultured on the surface for at least 10 passages, without any loss of pluripotency or karyotype changes. As a result, the device is adaptable for scalable cell expansion as cells grow directly on the polystyrene surface. The surface works in synergy with a ROCK inhibitor for expansion of human ESC, IPS, as well as human embryonic kidney cells (HEK) to enable enzymatic and mechanical passage protocols.