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Supermix

By R&D Editors | May 17, 2012

[[{“type”:”media”,”view_mode”:”media_original”,”fid”:”129794″,”attributes”:{“class”:”media-image”,”height”:”200″,”style”:”float: left; margin: 3px;”,”typeof”:”foaf:Image”,”width”:”250″}}]]Bio-Rad Laboratories Inc. has introduced two iTaq Universal supermixes that are compatible with any real-time PCR instrument. These products deliver robust performance and enable users to obtain high-quality data across all real-time quantitative PCR (qPCR) applications.

The two iTaq Universal supermixes include the iTaq Universal SYBR Green supermix and the iTaq Universal Probes supermix, both of which are 2x concentrated, ready-to-use reaction master mixes. Both supermixes are made with a proprietary optimized buffer formulation for maximum sensitivity, efficiency and reproducibility.

The term “universal” refers to the broad instrument compatibility and the ability to tolerate a broad range of reaction and protocol conditions. The iTaq Unversial supermixes, which contain a blend of multiple passive reference dyes, are particularly useful for researchers using real-time PCR instruments that require ROX fluorescent dyes. Researchers will now be able to use a single supermix, eliminating the need to change reagents between experiments and instruments.

The SYBR Green supermix is optimized for dye-based qPCR and contains SYBR® Green I dye whereas the Probes supermix is formulated for probe-based qPCR (simplex or duplex).

Key benefits of the iTaq Universal supermixes include:

• Broad dynamic range and sensitivity — offers accurate and sensitive detection of a broad range of targets in gene expression analysis and other real time applications
• Optimized buffer formulation — provides efficient amplification of genomic DNA templates and difficult amplicons
• Overcomes challenging duplex qPCR — enables detection of two gene targets, even with a great differential in copy number, in one reaction
• Antibody-mediated hot-start DNA polymerase — gives instant and complete polymerase activation in 30 seconds

Date: May 16, 2012
Source: Bio-Rad Laboratories Inc.

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