The two iTaq Universal supermixes include the iTaq Universal SYBR Green supermix and the iTaq Universal Probes supermix, both of which are 2x concentrated, ready-to-use reaction master mixes. Both supermixes are made with a proprietary optimized buffer formulation for maximum sensitivity, efficiency and reproducibility.
The term “universal” refers to the broad instrument compatibility and the ability to tolerate a broad range of reaction and protocol conditions. The iTaq Unversial supermixes, which contain a blend of multiple passive reference dyes, are particularly useful for researchers using real-time PCR instruments that require ROX fluorescent dyes. Researchers will now be able to use a single supermix, eliminating the need to change reagents between experiments and instruments.
The SYBR Green supermix is optimized for dye-based qPCR and contains SYBR® Green I dye whereas the Probes supermix is formulated for probe-based qPCR (simplex or duplex).
Key benefits of the iTaq Universal supermixes include:
• Broad dynamic range and sensitivity — offers accurate and sensitive detection of a broad range of targets in gene expression analysis and other real time applications
• Optimized buffer formulation — provides efficient amplification of genomic DNA templates and difficult amplicons
• Overcomes challenging duplex qPCR — enables detection of two gene targets, even with a great differential in copy number, in one reaction
• Antibody-mediated hot-start DNA polymerase — gives instant and complete polymerase activation in 30 seconds
Date: May 16, 2012
Source: Bio-Rad Laboratories Inc.