A new microscopy method could enable scientists to generate snapshots of dozens of different biomolecules at once in a single human cell, a team from the Wyss Institute of Biologically Inspired Engineering at Harvard Univ. reported in Nature Methods.

Such images could shed light on complex cellular pathways and potentially lead to new ways to diagnose disease, track its prognosis or monitor the effectiveness of therapies at a cellular level.

Cells often employ dozens or even hundreds of different proteins and RNA molecules to get a complex job done. As a result, cellular job sites can resemble a busy construction site, with many different types of these tiny cellular workers coming and going. Today’s methods typically only spot at most three or four types of these tiny workers simultaneously. But to truly understand complex cellular functions, it’s important to be able to visualize most or all of those workers at once, said Peng Yin, PhD, a core faculty member at the Wyss Institute and asst. prof. of systems biology at Harvard Medical School.

“If you can see only a few things at a time, you are missing the big picture,” Yin said.

Yin’s team sought a way to take aerial views of job sites that could spot up to dozens of types of biomolecules that make up large cellular work crews.

To capture ultra-sharp images of biomolecules, they had to overcome laws of physics that stymied microscopists for most of the last century. When two objects are closer than about 200 nm apart they can’t be distinguished using a traditional light microscope: The viewer sees one blurry blob where in reality there are two objects.

Since the mid-1990s, scientists have developed several ways to overcome this problem using combinations of specialized optics, special fluorescent proteins or dyes that tag cellular components.

Ralf Jungmann, PhD, now a postdoctoral fellow working with Yin at the Wyss Institute and Harvard Medical School, helped develop one of those super-resolution methods, called DNA-PAINT, as a graduate student. DNA-PAINT can create ultra-sharp snapshots of up to three cellular workers at once by labeling them with different colored dyes.

To visualize cellular job sites with crews of dozens of cellular workers, Yin’s team, including Jungmann, Maier Avendano, MS, a graduate student at Harvard Medical School, and Johannes Woehrstein, a postgraduate research fellow at the Wyss Institute, modified DNA-PAINT to create a new method called Exchange-PAINT.

Exchange-PAINT relies on the fact that DNA strands with the correct sequence of letters, or nucleotides, bind specifically to partner strands with complementary sequences. The researchers label a biomolecule they want to visualize with a short DNA tag, then add to the solution a partner strand carrying a fluorescent dye that lights up only when the two strands pair up. When that partner strand binds the tagged biomolecule, it lights up, then lets go, causing the biomolecule to “blink” at a precise rate the researchers can control. The researchers use this blinking to obtain ultra-sharp images.

They then repeat the process to visualize a second target, a third, and so on. Then they overlay the resulting images to create a composite image in which each biomolecule—each cellular worker—is assigned a different color. This allows them to create false-color images that simultaneously show many types of biomolecules—far more than they could simultaneously visualize by labeling them with different colored dyes. And these false-color images allow them to spot enough cellular workers at once to capture the entire scene.

To test Exchange-PAINT, the researchers created 10 unique pieces of folded DNA, or DNA origami, that resembled the numerals zero through nine. These numerals could be resolved with less than 10-nm resolution, or one-twentieth of the diffraction limit.

The team was able to use Exchange-PAINT to capture clear images of the 10 different types of miniscule DNA origami structures in one image. They also used the method to capture detailed, ultra-sharp images of fixed human cells, with each color tagging an important cellular component—microtubules, mitochondria, Golgi apparatus or peroxisomes.

Yin expects the method, with further development, to be able to visualize dozens of cellular components at once.

Source: Wyss Institute for Biologically Inspired Engineering at Harvard Univ.