Ivan Jayapurna, a UC Berkeley materials science and engineering graduate student, preparing a sample film of a new biodegradable plastic. (Credit: Adam Lau/UC Berkeley)
Despite our efforts to sort and recycle, less than 9% of plastic gets recycled in the U.S., and most end up in landfills or the environment.
Biodegradable plastic bags and containers could help, but if they’re not correctly sorted, they can contaminate otherwise recyclable #1 and #2 plastics. What’s worse, most biodegradable plastics take months to break down, and when they finally do, they form microplastics – tiny bits of plastic that can end up in oceans and animals’ bodies – including our own.
As reported today in the journal Nature, scientists at the Department of Energy’s Lawrence Berkeley National Laboratory (Berkeley Lab) and UC Berkeley have designed an enzyme-activated compostable plastic that could diminish microplastic pollution and holds great promise for plastic upcycling. The material can be broken down into its building blocks – small individual molecules called monomers – and then reformed into a new compostable plastic product.
“In the wild, enzymes are what nature uses to break things down – and even when we die, enzymes cause our bodies to decompose naturally. So for this study, we asked ourselves, ‘How can enzymes biodegrade plastic so it’s part of nature?” said senior author Ting Xu, who holds titles of faculty senior scientist in Berkeley Lab’s Materials Sciences Division and professor of chemistry and materials science and engineering at UC Berkeley.
At Berkeley Lab, Xu — who has dedicated her career to the development of functional polymer materials inspired by nature for nearly 15 years — is leading an interdisciplinary team of scientists and engineers from universities and national labs around the country to tackle the mounting problem posed by both single-use and so-called biodegradable plastics.
Most biodegradable plastics today are made of polylactic acid (PLA), a vegetable-based plastic material blended with cornstarch. Polycaprolactone (PCL) is also a biodegradable polyester widely used for biomedical applications such as tissue engineering.
Image of microplastics on the beach. (Credit: Shutterstock/Eric Dale)
But the problem with conventional biodegradable plastic is that they’re indistinguishable from single-use plastics such as plastic film – so a good chunk of these materials ends up in landfills. And even if a biodegradable plastic container gets deposited at an organic waste facility, it can’t break down as fast as the lunch salad it once contained, so it ends up contaminating organic waste, said co-author Corinne Scown, a staff scientist and deputy director for the Research, Energy Analysis & Environmental Impacts Division in Berkeley Lab’s Energy Technologies Area.
Another problem with biodegradable plastics is that they aren’t as strong as regular plastic – that’s why you can’t carry heavy items in a standard green compost bag. The tradeoff is that biodegradable plastics can break down over time – but still, Xu said, they only break down into microplastics, which are still plastic, just a lot smaller.
So Xu and her team decided to take a different approach – “nanoconfining” enzymes into plastics.
Putting enzymes to work
Because enzymes are part of living systems, the trick was carving out a safe place in the plastic for enzymes to lie dormant until they’re called to action.
In a series of experiments, Xu and her co-authors embedded trace amounts of the commercial enzymes Burkholderia cepacian lipase (BC-lipase) and proteinase K within PLA and PCL plastic materials. The scientists also added an enzyme protectant called four-monomer random heteropolymer, or RHP, to help disperse the enzymes a few nanometers (billionths of a meter) apart.
In a stunning result, the scientists discovered that ordinary household tap water or standard soil composts converted the enzyme-embedded plastic material into its monomers and eliminated microplastics in a few days or weeks.
They also learned that BC-lipase is a finicky “eater.” Before a lipase can convert a polymer chain into monomers, it must first catch the end of the chain. By controlling when the lipase finds the chain end, it is possible to ensure that the materials don’t degrade until triggered by hot water or compost soil, Xu explained.
In addition, they found that this strategy only works when BC-lipase is nano-dispersed – in this case, just 0.02% by weight in the PCL block – rather than randomly tossed in and blended.
“Nanodispersion puts each enzyme molecule to work – nothing goes to waste,” Xu said.
That matters when factoring in costs. Industrial enzymes can cost around $10 per kilogram, but this new approach would only add a few cents to the production cost of a kilogram of resin because the amount of enzymes required is so low — and the material has a shelf life of more than seven months, Scown added.
The proof is in the compost.
X-ray scattering studies performed at Berkeley Lab’s Advanced Light Source characterized the nano dispersion of enzymes in the PCL and PLA plastic materials.
Interfacial-tension experiments conducted by co-author Tom Russell revealed in real time how the size and shape of droplets changed as the plastic material decomposed into distinct molecules. The lab results also differentiated between enzyme and RHP molecules.
“The interfacial test gives you information about how the degradation is proceeding,” he said. “But the proof is in the composting – Ting and her team successfully recovered plastic monomers from biodegradable plastic simply by using RHPs, water, and compost soil.”
Russell is a visiting faculty scientist and professor of polymer science and engineering from the University of Massachusetts who leads the Adaptive Interfacial Assemblies Towards Structuring Liquids program in Berkeley Lab’s Materials Sciences Division.
Developing a very affordable and easily compostable plastic film could incentivize produce manufacturers to package fresh fruits and vegetables with compostable plastic instead of single-use plastic wrap. As a result, organic waste facilities would save the extra expense of investing in expensive plastic-depackaging machines when they want to accept food waste for anaerobic digestion or composting, Scown said.
Since their approach could work well with both hard, rigid plastics and soft, flexible plastics, Xu would like to broaden their study to polyolefins, a ubiquitous family of plastics commonly used to manufacture toys and electronic parts.
The team’s truly compostable plastic could be on the shelves soon. They recently filed a patent application through UC Berkeley’s patent office. Co-author Aaron Hall, who was a Ph.D. student in materials science and engineering at UC Berkeley at the time of the study, founded the UC Berkeley startup Intropic Materials to further develop the new technology. He was recently selected to participate in Cyclotron Road, an entrepreneurial fellowship program in partnership with Activate.
“When it comes to solving the plastics problem, it’s our environmental responsibility to take nature on its path. By prescribing a molecular map with enzymes behind the wheel, our study is a good start,” Xu said.
The paper’s co-authors include Christopher DelRe (lead author), Yufeng Jiang, Philjun Kang, Junpyo Kwon, Ivan Jayapurna, Zhiyuan Ruan, Le Ma, Kyle Zolkin, Tim Li, and Robert Ritchie.
The DOE Office of Science supported this work. DOE’s Laboratory Directed Research and Development program and the U.S. Department of Defense provided additional support.
The technology is available for licensing and collaboration. If interested, please contact Berkeley Lab’s Intellectual Property Office at [email protected].
More:
“New process makes ‘biodegradable’ plastics truly compostable,” UC Berkeley news release, published April 21, 2021
