Argonne National Laboratory and Yale University researchers have used cryo-electron microscopy (cryo-EM) to obtain a high-resolution structural view of a photosystem I (PSI)-platinum nanoparticle biohybrid catalyst. This finding provides critical insights into the design of efficient systems for solar-driven hydrogen production.
Background and context
Image courtesy of the Department of Energy Office of Science
Photosystem I (PSI) is a protein complex integral to the photosynthetic process, which converts sunlight into chemical energy. PSI exhibits near-unity quantum efficiency, generating one electron for nearly every photon absorbed. This characteristic makes it a good candidate for integrating into biohybrid systems for sustainable energy production.
Platinum nanoparticles, known for their catalytic properties, facilitate the reduction of protons to produce hydrogen gas. By combining PSI with platinum nanoparticles, researchers have created a biohybrid catalyst where the light absorbed by PSI drives the catalytic production of hydrogen.
High-resolution structural determination
Previous studies have demonstrated the functionality of PSI-platinum biohybrids. Still, the precise binding sites of the platinum nanoparticles on PSI remained unknown. Using cryo-EM, the research team identified two distinct binding sites for the nanoparticles on the PSI protein complex. This finding contradicts earlier assumptions that nanoparticle attachment occurs exclusively at electron transfer partner sites.
Implications for catalyst optimization
Identifying multiple binding sites provides a foundation for the rational design and optimization of biohybrid catalysts. Researchers can enhance the system’s catalytic efficiency by engineering specific interactions between PSI and platinum nanoparticles. Strategies may include modifying the PSI protein or nanoparticle properties to improve electron transfer and hydrogen production rates.
Research contributions and methodology
The structural characterization represents the culmination of 13 years of research. In earlier work, the team successfully demonstrated hydrogen production using PSI-based systems. The current study leverages cryo-EM to achieve sub-nanometer resolution of the biohybrid complex, enabling precise mapping of protein-nanoparticle interactions.
The research team includes Lisa M. Utschig, Christopher J. Gisriel, Tirupathi Malavath, Tianyin Qiu, Jan Paul Menzel, Victor S. Batista, and Gary W. Brudvig. The U.S. Department of Energy’s Office of Basic Energy Sciences and the National Institute of General Medical Sciences of the National Institutes of Health provided funding for this work.
Conclusion and future directions
The elucidation of PSI-platinum nanoparticle binding sites offers a pathway for engineering advanced biohybrid systems with improved functionality. Further research will focus on optimizing protein-nanoparticle interactions and scaling up these systems for practical applications in hydrogen fuel production.
The findings were published in Nature Communications.
