Bio-Rad Laboratories Inc. announces the launch of its Immun-Blot LF (low fluorescence) PVDF membranes, protein blotting membranes that are optimized for fluorescent and multiplex fluorescent applications. The membranes offer high signal-to-background ratio, low autofluorescence, and superior protein retention to maximize blot detection sensitivity and enable downstream quantitation.
There are two primary types of protein blotting membranes used in Western blotting applications: Nitrocellulose and PVDF (polyvinylidene fluoride). While both membrane types are suitable for colorimetric and chemiluminescent detection, their high autofluorescence and lower signal-to-background ratios are not suitable for fluorescent detection.
Immun-Blot LF PVDF membranes are a low fluorescent alternative specifically designed for fluorescent immunoblotting and detection in the visible light (Red/Green/Blue) spectrum. Immun-Blot LF PVDF membranes produce highly sensitive and quantitative results due to superior signal-to-background ratios, which are higher than low fluorescence membrane solutions (Bulletin 6116) and are three times higher than nitrocellulose and conventional PVDF.
• Low autofluorescence and high signal-to-background ratio — produces a stronger signal, more accurate protein quantitation, and higher quality images
• Superior protein retention — offers binding capacity of 300 µg/cm2 and improves the ability to capture more proteins, especially low-abundance proteins
• Precut membrane and filter paper sets — saves time and eliminates excessive membrane handling and potential contamination
Immun-Blot LF PVDF membranes are compatible with any imager possessing visible RGB excitation channels, including Bio-Rad’s new ChemiDoc MP imager, which allows researchers to visualize proteins at every stage of their blotting experiment when paired with Bio-Rad’s proprietary Stain-Free technology. The membranes are also compatible with a wide range of fluorescent labels such as DyLights, Q-Dots, and Alexa fluorophores as well as colorimetric and chemiluminescent detection methods.
