GE Healthcare’s rProtein A GraviTrap, Protein G GraviTrap and rProtein A/Protein G GraviTrap, gravity-flow columns enable single-step, efficient manual purification of monoclonal and polyclonal antibodies from various species in milligram scale. The high capacity columns deliver high purity purification with a run time of approximately 30 minutes (dependent on sample).
These columns provide reliable, easy-to-use solutions for small-scale antibody purification. The product packaging contains everything that is needed for manual purification, as it readily converts into a convenient column stand (Workmate) with a plastic tray to collect liquid waste.
Each pack contains 10 prepacked columns to facilitate multiple samples to be purified simultaneously, using a gravity format. Columns contain 1 ml bed volume and can bind milligram amounts of antibodies. The columns are reusable up to five times depending on the sample.
With high affinity for various species of antibodies, the media used are:
• rProtein A GraviTrapcontains rProtein A Sepharose FF
• Protein G GraviTrap contains Protein G Sepahrose 4 FF
• rProtein A/Protein G GraviTrap contains 50% rProtein A Sepharose FF and 50% Protein G Sepharose 4 FF